What happens if you increase the amount of enzyme

What we expect to see is that the rate should level off once it reaches the maximum when every peroxidase enzyme has a hydrogen peroxide molecule to interact with -- at that point, adding more hydrogen peroxide can't increase the reaction rate because all of the available enzymes are busy. Effects of Enzyme Concentration. We also examined reaction rate when we increased the amount of turnip extract in our tubes. Since the turnip extract contained our peroxidase enzyme, this increased the enzyme concentration in our reactions.

The graph above shows that as we increased the amount of enzyme in the tubes, our reaction rate increased. At some point, the maximum raction rate should be achieved and we should see the curve level off see the substrate concentration curve above for an example , but based on this data, more than 3 mL of turnip extract is required to reach a ratio of enzyme to substrate molecules. What properties affect the slope of this equation?

Sources: Michaelis, L. Die kinetik der invertinwirkung. Segel, L. Modeling dynamic phenomena in molecular and cellular biology. Cambridge University Press, NY. Darnell, J. Lodish, and D. Molecular cell biology. Each enzyme work bests at a specific pH value. The optimum pH for an enzyme depends on where it normally works. For example, enzymes in the small intestine have an optimum pH of about 7.

In the graph above, as the pH increases so does the rate of enzyme activity. Graphically this can be represented as:. Figure 1. Zero-order reaction rate is dependent of substrate concentration. These reactions are said to be "zero-order reactions" because the rates are independent of substrate concentration.

The formation of product proceeds at a rate which is linear with time. The addition of more substrate does not serve to increase the rate. In zero-order kinetics, allowing the assay to run for double time results in double the amount of product. The amount of enzyme present in a reaction is measured by the activity it catalyzes. The relationship between activity and concentration is affected by many factors such as temperature, pH, etc.